Adaptation of the AOCS official method for measuring hydroperoxides from small-scale oil samples

Abstract  An adaptation of the American Oil Chemists' Society Official Method Cd 8–53 for determining peroxides in fats and oils using a 0.5-g sample is described. Comparisons of the Official Method and the small-scale method were performed by analyzing soybean oil samples spiked with t-butyl hydroperoxide and autoxidized soybean oil samples. A linear relationship between the Official Method and the small-scale method was obtained with an R 2 of 0.998. The small-scale method is sensitive, precise, and suitable for small sample sizes and uses only about 10% of the chemicals necessary for the Official Method.